Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.
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Open in a separate window. The polymerase chain reaction PCR is a rapid tool that can be used no only to detect acutely PRV infected pigs but it is the recommended test for detect PRV latent infection. The nucleotide sequence amplified aujfszky this study corresponds to a bp fragment in the gD gene of the PRV genome The assay proved to be very sensitive due to deona little as 1. This was possible due to the high annealing temperature of the primers pair designed and contributes to the reaction efficiency.
All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License. Replication in the respiratory tract, central nervous system and reproductive organs is responsible for pathological changes causing different disorders Journal List Braz J Microbiol v.
The analytical sensitivity of the test was estimated to be 1.
Articles from Brazilian Journal of Microbiology are provided here courtesy of Elsevier. Potential sites of virus latency associated with indigenous pseudorabies viruses in feral swine.
Oligonucleotide primers and restriction endonuclease selection PRV specific primers were designed using the Oligo 6. The analysis directly from clinical samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases. Each one of the nine tissue field samples from pigs diagnosed as PRV infected based on clinical signs and laboratory methods yielded the corresponding PRV amplified product when analyzed.
The analytical sensitivity of the test was consistently observed to be 1. Under typical conditions of intensive swine production, several clinically similar viral diseases can occur which require laboratory differential diagnosis. Table 1 Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome. A rapid, sensitive and specific PCR-based diagnostic assay to detect pseudorabies virus in clinical samples is provided.
Can J Com Med. The polymerase chain reaction PCR can be used to identify PRV genomes in secretions or organ samples and although some PCR assays for PRV detection with different sensitivities have been reported 37915 there is no aujeszyk procedure recommended so far 2. Published online Sep 1. PRV specific primers were designed using the Oligo 6.
Doença de Aujeszky – Wikipédia, a enciclopédia livre
Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and in pigs with emphasis on the respiratory tract. Support Center Support Center. This assay was based on the amplification of a highly conserved viral gD gene fragment.
The isolation and characterization of a strain of infectious bovine rhinotracheitis virus from stillbirth in swine. This paper describes the development, optimization and performance assessment of a rapid and highly sensitive PCR test for detection of pseudorabies virus.
National Center for Biotechnology InformationU. Also, the BLAST search against nucleotide databases of different herpesvirus and random nucleotide sequences revealed aujeszkh region is very specific for PRV genomes.
The Sma I restriction endonuclease site was used for additional specificity confirmation of the amplification products. Iowa State University Press; Diagnostic ayjeszky for detection of Classical swine fever virus—Status quo and new developments. PCR experiments were performed on serial ten-fold dilutions of aujeszy viral suspension of PRV isolate Aujeeszky with a titer of 10 6. Agarose gel electrophoresis was used to detect PCR products.
Seven tissue samples from clinically healthy animals were negative for PCR amplification data not shown. Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus from pigs with postweaning multisystemic wasting syndrome by multiplex PCR. The PCR for PRV genome detection is also an important method in screening pig specimens collected for xenotransplantation to increase the safety of organ transplantation 7 and to detect viral infection in a wide spectrum of species reported to be susceptible to PRV, through either natural or experimental infections 8.
Doença de Aujeszky
This region was highly conserved for all reported genomes as shown by aligning of these sequences. Seven virus-negative tissues samples from clinically healthy animals were also included. Specificity of the PRV amplicons was furthermore confirmed by Sma I restriction endonuclease analysis which generated the two expected fragments of and bp in length Fig.
The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions from latently infected animals. This gene codes for an envelope glycoprotein named gD which plays and important role in binding cellular receptors and is critical for virus replication in different organs The analysis of tissue homogenate samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases.
Traditionally, PRV detection is based on direct virus isolation followed by confirmation using immunofluorescence, immunoperoxidase or neutralization tests with specific antiserum 2.